About Grid Crossing Microfilm
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About Grid Crossing Microfilm video introduction
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6 FAQs about [Grid Crossing Microfilm]
What is Micropatterning of cryo-EM grids?
Micropatterning of cryo-EM grids enables controlled adhesion of mammalian cells for cryo-ET-based structural studies. This approach leads to reproducible cellular morphology and improves focused ion beam thinning of cells for in-cell structural analyses.
Can go grids be functionalized for single-particle cryo-EM?
Functionalization of these GO grids with single-stranded DNA (ssDNA) or an amphiphilic polymer with comparable structure and charge properties leads to stabilization of nucleosome-protein complexes for single-particle cryo-EM (Fig. 1a).
Do functionalized graphene-oxide grids enable high-resolution cryo-EM structures without crosslinking?
Chio, U.S., Palovcak, E., Smith, A.A.A. et al. Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking.
Can a grid be used to analyze protein-nucleic acid complexes in cryoEM?
We envision these grids will enable similar detailed structural analyses for other enzyme-nucleosome complexes and possibly other protein-nucleic acid complexes in general. Nucleosome-protein complexes stick to the air-water interface and denature upon plunge freezing for cryoEM.
Does scanning transmission electron microscopy (STEM) affect GB migration?
Although scanning transmission electron microscopy (STEM) has a strong capability to directly image the static atomic structures of GBs 3, the poor temporal resolution limits the dynamic observations of GB migration.
Who developed a polymer-functionalized go grid?
U.S.C. performed subsequent cryo-EM data analysis with guidance from J.P.A. and Y.C. U.S.C. collected and analyzed tomography data. E.P. and H.A. conceived the idea for polymer-functionalized GO grids. A.A.A.S. synthesized TAASTY copolymer. F.W. synthesized GO, and F.W. and D.A. provided support on use of GO and on functionalization.